[article]
| Title : |
Hatchery hygiene mapping based on microbial load assessment in Chitwan, Nepal |
| Material Type: |
printed text |
| Authors: |
R.K. Bhattarai, Author |
| Publication Date: |
2017 |
| Article on page: |
161-169 p. |
| Languages : |
English (eng) |
| Keywords: |
commercial hatchery, poultry, contamination, sample |
| Abstract: |
A study from March to August, 2016 was conducted to understand the level of hatchery hygiene in
Chitwan using questionnaire survey and microbial load assessment. Key points of contact during
chicks production were selected and contact plate samples (n=220) were collected aseptically
from ten representative commercial hatcheries. Samples were inoculated and incubated in
Nutrient Agar and Mac Conkey Agar for 24 hrs and in Sabouraud Dextrose Agar for 48 hrs at
370
C. It was observed that biosecurity measures of disinfection, regulated entry, isolated setting
of eggs for layers, broilers and duck chick production and separate set of clothing for different
units were common practices in those hatcheries. The most cited areas for disinfection were unit
floor followed by hatchery walls. Bacterial and fungal load (CFU/90mm diameter petriplate) was
significantly (P
characteristics, most of the organisms were gram-negative motile rods indicative of E. coli and
Salmonella with a few gram-positives and Aspergillus species. All hatchery units had higher load
of bacteria and fungi, indicating less effective biosecurity and hygiene. An improved hygienic
practice is recommended in the hatcheries in Chitwan. |
| Link for e-copy: |
https://afu.edu.np/sites/default/files/Hatchery_hygiene_mapping_based_on_microbi [...] |
in Journal of Agriculture and Forestry University > Volume 1 (2017) . - 161-169 p.
[article] Hatchery hygiene mapping based on microbial load assessment in Chitwan, Nepal [printed text] / R.K. Bhattarai, Author . - 2017 . - 161-169 p. Languages : English ( eng) in Journal of Agriculture and Forestry University > Volume 1 (2017) . - 161-169 p.
| Keywords: |
commercial hatchery, poultry, contamination, sample |
| Abstract: |
A study from March to August, 2016 was conducted to understand the level of hatchery hygiene in
Chitwan using questionnaire survey and microbial load assessment. Key points of contact during
chicks production were selected and contact plate samples (n=220) were collected aseptically
from ten representative commercial hatcheries. Samples were inoculated and incubated in
Nutrient Agar and Mac Conkey Agar for 24 hrs and in Sabouraud Dextrose Agar for 48 hrs at
370
C. It was observed that biosecurity measures of disinfection, regulated entry, isolated setting
of eggs for layers, broilers and duck chick production and separate set of clothing for different
units were common practices in those hatcheries. The most cited areas for disinfection were unit
floor followed by hatchery walls. Bacterial and fungal load (CFU/90mm diameter petriplate) was
significantly (P
characteristics, most of the organisms were gram-negative motile rods indicative of E. coli and
Salmonella with a few gram-positives and Aspergillus species. All hatchery units had higher load
of bacteria and fungi, indicating less effective biosecurity and hygiene. An improved hygienic
practice is recommended in the hatcheries in Chitwan. |
| Link for e-copy: |
https://afu.edu.np/sites/default/files/Hatchery_hygiene_mapping_based_on_microbi [...] |
|
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