Author details
Author B. Devkota |
Available item(s) by this author (7)
Refine your search Apply to external sourcesAssociation of nutritional status to reproductive performance in buffaloes / B. Devkota in Journal of Agriculture and Forestry University, Volume 2 (2018)
Title : Association of nutritional status to reproductive performance in buffaloes Material Type: printed text Authors: B. Devkota, Author Publication Date: 2018 Article on page: 1-7 p. Languages : English (eng) Keywords: Anestrus Buffalo Nutrition Reproduction Season Link for e-copy: http://afu.edu.np/sites/default/files/Association_of_nutritional_status_to_repro [...]
in Journal of Agriculture and Forestry University > Volume 2 (2018) . - 1-7 p.[article] Association of nutritional status to reproductive performance in buffaloes [printed text] / B. Devkota, Author . - 2018 . - 1-7 p.
Languages : English (eng)
in Journal of Agriculture and Forestry University > Volume 2 (2018) . - 1-7 p.
Keywords: Anestrus Buffalo Nutrition Reproduction Season Link for e-copy: http://afu.edu.np/sites/default/files/Association_of_nutritional_status_to_repro [...]
Title : Current diagnostic techniques of Mycobacterium avium sub sp. paratuberculosis in domestic ruminants Material Type: printed text Authors: S. Singh, Author ; I. P. Dhakal, Author ; U. M. Singh, Author ; B. Devkota, Author Publication Date: 2018 Article on page: 23-34 p. Languages : English (eng) Keywords: Paratuberculosis, molecular, culture, ELISA, PCR, MAP Abstract: Mycobacterium avium subspecies paratuberculosis (MAP) causes Johne’s disease (JD), a chronic
wasting disease in cattle with important welfare, economic and potential public health implications.
Current laboratory tests are unable to recognize all stages of the disease, which makes it difficult to
diagnose and control the disease. Methods such as fecal smear, acid-fast stain, bacterial culture and
polymerase chain reaction (PCR) tests are used as direct tests while detection of host response that
includes clinical signs in combination with gross and microscopic pathology and immunologic markers
of infection that include antibody response to MAP (serology), delayed-type hypersensitivity (DTH)
reaction, lymphocyte proliferation, and increased cytokine (IFN-γ) production- are the indirect ways
to diagnose MAP infection. For effective control, early and confirmatory diagnosis is quite important.
Despite considerable research effort, all methods are fraught with difficulties that have impeded the
control and eradication of paratuberculosis. This article briefly reviews the recent diagnostic tests
available for diagnosis of MAP which can facilitate clinician’s ability to apply the results and evaluate
the test sensitivity and specificity to compare the accuracy of different tests in detecting MAP.
Link for e-copy: https://afu.edu.np/sites/default/files/Current_diagnostic_techniques_of_Mycobact [...]
in Journal of Agriculture and Forestry University > Volume 2 (2018) . - 23-34 p.[article] Current diagnostic techniques of Mycobacterium avium sub sp. paratuberculosis in domestic ruminants [printed text] / S. Singh, Author ; I. P. Dhakal, Author ; U. M. Singh, Author ; B. Devkota, Author . - 2018 . - 23-34 p.
Languages : English (eng)
in Journal of Agriculture and Forestry University > Volume 2 (2018) . - 23-34 p.
Keywords: Paratuberculosis, molecular, culture, ELISA, PCR, MAP Abstract: Mycobacterium avium subspecies paratuberculosis (MAP) causes Johne’s disease (JD), a chronic
wasting disease in cattle with important welfare, economic and potential public health implications.
Current laboratory tests are unable to recognize all stages of the disease, which makes it difficult to
diagnose and control the disease. Methods such as fecal smear, acid-fast stain, bacterial culture and
polymerase chain reaction (PCR) tests are used as direct tests while detection of host response that
includes clinical signs in combination with gross and microscopic pathology and immunologic markers
of infection that include antibody response to MAP (serology), delayed-type hypersensitivity (DTH)
reaction, lymphocyte proliferation, and increased cytokine (IFN-γ) production- are the indirect ways
to diagnose MAP infection. For effective control, early and confirmatory diagnosis is quite important.
Despite considerable research effort, all methods are fraught with difficulties that have impeded the
control and eradication of paratuberculosis. This article briefly reviews the recent diagnostic tests
available for diagnosis of MAP which can facilitate clinician’s ability to apply the results and evaluate
the test sensitivity and specificity to compare the accuracy of different tests in detecting MAP.
Link for e-copy: https://afu.edu.np/sites/default/files/Current_diagnostic_techniques_of_Mycobact [...]
Title : Detection of Mycobacterium avium sub sp. paratuberculosis (MAP) by PCR in the faeces of dairy cattle of Chitwan, Nepal Material Type: printed text Authors: S. Singh, Author ; I. P. Dhakal, Author ; U. M. Singh, Author ; B. Devkota, Author Publication Date: 2020 Article on page: 217-224 p. Languages : English (eng) Keywords: Johne's disease, Diagnosis, Mycobacterium avium subsp. paratuberculosis, Faecal culture, fPCR,Nepal. Abstract: Johne's disease or Mycobacterium avium subspecies paratuberculosis (MAP) causes chronic granulomatous enteritis with
decrease in production resulting huge economic losses with high negative impact on the livestock industry. Diagnosis of
MAP is difficult due to lack of characteristics clinical signs, prolong incubation period in cultivation of MAP, and nonspecific results in diagnostic tests. To the best of our knowledge there is no report on faecal culture and molecular detection of
MAP in dairy cattle of Nepal. The main objective of this research was to access the herd level prevalence of Johne’s disease
in the representative dairy farms of Chitwan district with the use of modern techniques as faecal polymerase chain reaction
(fPCR) to know the MAP distribution in dairy cattle. A total of 265 individual dairy cattle faeces sample were collected
during February 2017 to January 2018 from dairy farms of three different geographical location of Chitwan district, Nepal.
Faeces were decontaminated and subjected for faecal culture as well as fPCR to have molecular detection of MAP. Findings
revealed that bio-load of MAP in dairy cattle were 13.57% by faecal culture, and 16.59% by fPCR detection method. The
overall prevalence of MAP in dairy cattle was detected as 16.59 % by fPCR. Likewise, IS900 PCR assay proved to be a more
sensitive and reliable test than faecal culture for the detection of MAP in faecal sample of clinically suspected dairy cattle
as the PCR assay was able to detect significantly (p suggests that IS900-PCR-based detection of MAP could be used as a potential diagnostic tool for rapid and effective Johne’s
disease (JD) surveillance as compared with faecal culture detection method due to its advantage for JD control programs by
reducing the time of definitive diagnosis from several months to a few days. This is the first molecular level of diagnostic
research performed and reporting of MAP in dairy cattle of Nepal. These results will be useful in designing suitable disease
control strategy for livestock industry.Link for e-copy: https://afu.edu.np/sites/default/files/Detection%20of%20Mycobacterium%20avium%20 [...]
in Journal of Agriculture and Forestry University > Volume 4 (2020) . - 217-224 p.[article] Detection of Mycobacterium avium sub sp. paratuberculosis (MAP) by PCR in the faeces of dairy cattle of Chitwan, Nepal [printed text] / S. Singh, Author ; I. P. Dhakal, Author ; U. M. Singh, Author ; B. Devkota, Author . - 2020 . - 217-224 p.
Languages : English (eng)
in Journal of Agriculture and Forestry University > Volume 4 (2020) . - 217-224 p.
Keywords: Johne's disease, Diagnosis, Mycobacterium avium subsp. paratuberculosis, Faecal culture, fPCR,Nepal. Abstract: Johne's disease or Mycobacterium avium subspecies paratuberculosis (MAP) causes chronic granulomatous enteritis with
decrease in production resulting huge economic losses with high negative impact on the livestock industry. Diagnosis of
MAP is difficult due to lack of characteristics clinical signs, prolong incubation period in cultivation of MAP, and nonspecific results in diagnostic tests. To the best of our knowledge there is no report on faecal culture and molecular detection of
MAP in dairy cattle of Nepal. The main objective of this research was to access the herd level prevalence of Johne’s disease
in the representative dairy farms of Chitwan district with the use of modern techniques as faecal polymerase chain reaction
(fPCR) to know the MAP distribution in dairy cattle. A total of 265 individual dairy cattle faeces sample were collected
during February 2017 to January 2018 from dairy farms of three different geographical location of Chitwan district, Nepal.
Faeces were decontaminated and subjected for faecal culture as well as fPCR to have molecular detection of MAP. Findings
revealed that bio-load of MAP in dairy cattle were 13.57% by faecal culture, and 16.59% by fPCR detection method. The
overall prevalence of MAP in dairy cattle was detected as 16.59 % by fPCR. Likewise, IS900 PCR assay proved to be a more
sensitive and reliable test than faecal culture for the detection of MAP in faecal sample of clinically suspected dairy cattle
as the PCR assay was able to detect significantly (p suggests that IS900-PCR-based detection of MAP could be used as a potential diagnostic tool for rapid and effective Johne’s
disease (JD) surveillance as compared with faecal culture detection method due to its advantage for JD control programs by
reducing the time of definitive diagnosis from several months to a few days. This is the first molecular level of diagnostic
research performed and reporting of MAP in dairy cattle of Nepal. These results will be useful in designing suitable disease
control strategy for livestock industry.Link for e-copy: https://afu.edu.np/sites/default/files/Detection%20of%20Mycobacterium%20avium%20 [...]
Title : Morphometry of the female reproductive organs of the Murrah cross buffaloes in Chitwan, Nepal Material Type: printed text Authors: B. Devkota, Author ; A. K. Singh, Author Publication Date: 2017 Article on page: 113-117 p. Languages : English (eng) Keywords: buffalo, reproductive organs Abstract: The study was conducted to understand the normal morphometry of reproductive organs of Nepalese
Murrah cross buffaloes. Reproductive organs of mature buffaloes (n=41) were collected from local
slaughter house and normal samples (n=35) were used to determine the normal morphometry.
Three diamentional measurements of the ovaries, structures within the ovaries, such as corpus
luteum (CL) and follicles (F) and lengths and diameters of the cervix, uterine body and uterine
horns were measured by using standard measurement techniques. Ovaries were categorized into
4 types on the basis of the presence or absence of one or both functional structures of CL and
F ≥ 0.8 cm. The size of the reproductive organs of these buffaloes was almost similar with, or
little smaller, to that reported for other breeds. The largest CL length and follicular diameter was
1.8 and 1.4 cm, respectively. There were significant correlations (r ≥ 0.56, p lengths, breadths, thickness and volumes of the ovaries. The overall size of the ovary was related
to the presence or absence of CL and F≥0.8 cm. The information documented in this study can be
valuable while dealing with the clinical reproductive disorders, performing rectal examination,
using transrectal ultrasonography and applying reproductive technologies in Nepalese buffaloes.Link for e-copy: https://afu.edu.np/sites/default/files/Morphometry_of_the_female_reproductive_or [...]
in Journal of Agriculture and Forestry University > Volume 1 (2017) . - 113-117 p.[article] Morphometry of the female reproductive organs of the Murrah cross buffaloes in Chitwan, Nepal [printed text] / B. Devkota, Author ; A. K. Singh, Author . - 2017 . - 113-117 p.
Languages : English (eng)
in Journal of Agriculture and Forestry University > Volume 1 (2017) . - 113-117 p.
Keywords: buffalo, reproductive organs Abstract: The study was conducted to understand the normal morphometry of reproductive organs of Nepalese
Murrah cross buffaloes. Reproductive organs of mature buffaloes (n=41) were collected from local
slaughter house and normal samples (n=35) were used to determine the normal morphometry.
Three diamentional measurements of the ovaries, structures within the ovaries, such as corpus
luteum (CL) and follicles (F) and lengths and diameters of the cervix, uterine body and uterine
horns were measured by using standard measurement techniques. Ovaries were categorized into
4 types on the basis of the presence or absence of one or both functional structures of CL and
F ≥ 0.8 cm. The size of the reproductive organs of these buffaloes was almost similar with, or
little smaller, to that reported for other breeds. The largest CL length and follicular diameter was
1.8 and 1.4 cm, respectively. There were significant correlations (r ≥ 0.56, p lengths, breadths, thickness and volumes of the ovaries. The overall size of the ovary was related
to the presence or absence of CL and F≥0.8 cm. The information documented in this study can be
valuable while dealing with the clinical reproductive disorders, performing rectal examination,
using transrectal ultrasonography and applying reproductive technologies in Nepalese buffaloes.Link for e-copy: https://afu.edu.np/sites/default/files/Morphometry_of_the_female_reproductive_or [...]
Title : Nutritional parameters in relation to reproductive performance in anestrus chauri (Yak hybrid) cattle around Jiri, Dolakha Material Type: printed text Authors: B. P. Gautam, Author ; B. Devkota, Author ; R. C. Sapkota, Author ; G. Gautam, Author ; S. K. Shah, Author Publication Date: 2018 Article on page: 165-169 p. Languages : English (eng) Keywords: BCS, chauri, pregnancy, vitamin-mineral supplementation Abstract: Anestrus Chauri (Yak hybrid) cattle raised in Jiri area of Dolakha district, Nepal were selected to
investigate the status of blood nutritional parameters in relation to their reproductive performance
during active breeding months. Blood samples were collected from 20 (13 pregnant and 7 nonpregnant) anestrus Chauries to estimate nutritional parameters in serum, and body condition score
(BCS) of each animal was recorded. The non-pregnant anestrus Chauries were supplemented with
vitamin-mineral mixture for two months followed by second blood sampling and BCS recording.
The response of supplementation was evaluated in terms of estrus, fertile mating and pregnancy. Four
out of 7 (57%) non-pregnant anestrus Chauries responded to the vitamin-mineral supplementation to
produce pregnancy. Statistical analysis revealed that level of blood glucose, total cholesterol, calcium
and phosphorus were not significantly different between pregnant and non-pregnant anestrus Chauries
while the level of serum total protein was significantly higher in pregnant than in non-pregnant
Chauries. BCS of pregnant and non-pregnant Chauries differed significantly (p conception was observed in Chauries with BCS greater than 3.0. When compared with the levels
of blood nutritional parameters before supplementation, higher level (p cholesterol and calcium were observed after vitamin-mineral supplementation. In conclusion, blood
nutritional parameters and BCS indicate the reproductive performance in Chauries, and vitaminmineral supplementation in non-pregnant Chauries helps in achieving pregnancy.Link for e-copy: https://afu.edu.np/sites/default/files/Nutritional_parameters_in_relation_to_rep [...]
in Journal of Agriculture and Forestry University > Volume 2 (2018) . - 165-169 p.[article] Nutritional parameters in relation to reproductive performance in anestrus chauri (Yak hybrid) cattle around Jiri, Dolakha [printed text] / B. P. Gautam, Author ; B. Devkota, Author ; R. C. Sapkota, Author ; G. Gautam, Author ; S. K. Shah, Author . - 2018 . - 165-169 p.
Languages : English (eng)
in Journal of Agriculture and Forestry University > Volume 2 (2018) . - 165-169 p.
Keywords: BCS, chauri, pregnancy, vitamin-mineral supplementation Abstract: Anestrus Chauri (Yak hybrid) cattle raised in Jiri area of Dolakha district, Nepal were selected to
investigate the status of blood nutritional parameters in relation to their reproductive performance
during active breeding months. Blood samples were collected from 20 (13 pregnant and 7 nonpregnant) anestrus Chauries to estimate nutritional parameters in serum, and body condition score
(BCS) of each animal was recorded. The non-pregnant anestrus Chauries were supplemented with
vitamin-mineral mixture for two months followed by second blood sampling and BCS recording.
The response of supplementation was evaluated in terms of estrus, fertile mating and pregnancy. Four
out of 7 (57%) non-pregnant anestrus Chauries responded to the vitamin-mineral supplementation to
produce pregnancy. Statistical analysis revealed that level of blood glucose, total cholesterol, calcium
and phosphorus were not significantly different between pregnant and non-pregnant anestrus Chauries
while the level of serum total protein was significantly higher in pregnant than in non-pregnant
Chauries. BCS of pregnant and non-pregnant Chauries differed significantly (p conception was observed in Chauries with BCS greater than 3.0. When compared with the levels
of blood nutritional parameters before supplementation, higher level (p cholesterol and calcium were observed after vitamin-mineral supplementation. In conclusion, blood
nutritional parameters and BCS indicate the reproductive performance in Chauries, and vitaminmineral supplementation in non-pregnant Chauries helps in achieving pregnancy.Link for e-copy: https://afu.edu.np/sites/default/files/Nutritional_parameters_in_relation_to_rep [...]
Permalink
Permalink
